低分子量南瓜多糖的提取、纯化及结构初步研究

Study on extraction,purification and structure of low molecular weight polysaccharides from pumpkin

采用水浸提法提取低分子量南瓜粗多糖。通过DEAE Sepharose Fast Flow离子交换层析和sephacryl s-100HR凝胶过滤层析得到南瓜低分子量多糖(LWPP-Ia),通过高效液相色谱测定了LWPP-Ia的纯度,显示为单一峰,分子量为6267Da。运用高效液相离子色谱测定了单糖组成,为葡萄糖(27.75%)、果糖(7.70%)、半乳糖(9.12%)。紫外扫描显示无蛋白、核酸杂质。红外光谱鉴定表明含有多糖的特征吸收峰,并含有吡喃糖环。

The raw polysaccharide was retained by water extracting. Using DEAE Sepharose Fast Flow ion exchange chromatography and Sephacryl S- 100 HR gel filtration chromatography （ GFC）, low molecular weight polysaccharides from pumpkin（ LWPP-la ）was got. Its purifity was confirmed by HPLC and was shown to be single symmetric peak. The molecular weight was 6267Da by HPLC. The monosaccharide composition of LWPP- la were also tested. In the acid hydrolysates of LWPP-la,analysis by HPLC showed the ratio of glucose,fructose, galactose was 27.75% ,7.70% ,9.12%. UV spectra measurements confirmed there was no protein and nucleic acid. IR spectra measurements showed it has the characteristic of glycoconjugate and contained pyranride.