Methylomonas Z201 细胞在戊二醛活化DEAE纤维素上的共价固定化

Covalently Immobilization of Methylomonas Z201 Cells on Glutaraldehyde Activated DEAE Cellulose

以戊二醛活化的ＤＥＡＥ纤维素为载体，采用共价键法固定化ＭｅｔｈｙｌｏｍｏｎａｓＺ２０１细胞．固定化细胞催化丙烯环氧化的最优细胞／载体比为３２ｍｇ干重细胞／（ｇＤＥＡＥ纤维素），最适ｐＨ值和最适温度分别为７．８和３５℃．用纯氧代替空气作为氧源，分别使游离和固定化细胞的ＭＭＯ活性提高了８％和３５％．外源性电子给体甲酸钠对固定化细胞ＭＭＯ活性的激发作用小于游离细胞．失活动力学分析结果表明，固定化细胞的稳定性高于游离细胞．

Methylomonas Z201 cells have been immobilized on the glutaraldehyde activated DEAE cellulose for the propene epoxidation. The optimum cell to carrier ratio was determined to be 32 mg dry weight cells/g DEAE cellulose. The optimum pH and the optimum temperature of covalently immolilized cells were 7.8 and 40℃ respectively. The MMO activities of free and covalently immobilized cells were increased by 8% and 35% respectivily, when the air was replaced by pure oxygen as the oxygen source. The activation effect of sodium formate on the MMO activity of covalently immobilized cells was less than that of free cells due to the presence of the carrier. The deactivation kinetics indicated that covalently immobilization enhanced the stability of the cells.