向日葵异戊烯焦磷酸异构酶基因(ipi)的电子克隆和生物信息学分析

Electronic Cloning and Characterizations of ipi Gene from Helianthus annus L.Using Bioinformatics Tools

用电子克隆方法获得向日葵异戊烯焦磷酸异构酶(IPI)基因,并用生物信息学方法对此基因编码产物从氨基酸组成、理化性质、二级和三级结构以及功能进行预测和分析的结果表明,向日葵IPI为亲水性α/β蛋白,含有IPP异构酶结构域,其与其他植物的IPI在序列组成、结构及活性位点均有高度的相似性。

An isopentenyl diphosphate isomerase （IPP） gene from Helianthus annus was cloned by electronic cloning based on the EST sequences from UniGene of NCBI. Some characters of the ipi deduced amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects： the composition of amino acid sequences, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein and function, and so on. Analysis results showed that ipi gene contained an IPP isomerase domain, belonging to α/β protein, despite sequence variations （mainly at the N-terminus）, the core structure, sequence and activity sites were highly similar with other plant IPI.