摘要
为探索构建组织工程脂肪的新方法,分离出转染GFP基因的小鼠脂肪间充质干细胞(ADSCs),将细胞、胶原结合碱性成纤维细胞生长因子(bFGF)的复合物注射到裸鼠皮下,6周后取材。试验组ADSCs经过2周成脂诱导,对照组ADSCs未经成脂诱导。组织学结果显示试验组形成典型的"网状"脂肪组织结构,对照组无明显的脂肪结构形成。RT-PCR证实试验组PPARγ2和C/EBPα基因的表达,对照组PPARγ2和C/EBPα基因不表达。ADSCs与bFGF混合,以胶原作为支架构建组织工程脂肪是可行的,为临床脂肪组织缺陷的治疗提供新的思路。
The objective of this research was to find a new method to reconstruct adipose tissue engineering. Mesenchymal stem cells derived from adipose tissue (ADSCs), harvested from transgenic GFP C57BL/6 mice, cultured in adipogenic differentiation medium at 37℃ and 5% CO2 for 2 weeks, suspended in collagen matrix mixed with basic fibroblast growth factor (bFGF) were injected to the subcutaneous of athymic mice for 6 weeks. Group of ADSCs without adipogenic differentiation was used as control. Histological analyses of the implants showed significant regeneration of adipose tissues. Abundant mature adipocytes in the retrieved tissues were stained positively with oil red O staining. RT-PCR analysis for PPAR72 and C/EBP α conformed to Oil red O staining. In contrast, no adipose tissue formation was found in the control group. This novel method to reconstruct adipose tissue engineering may be useful for treatment of adipose tissues traumas.
出处
《西北农业学报》
CSCD
北大核心
2008年第2期59-63,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
“863计划”组织器官工程重大专项(2002AA205041,2005AA205241)
关键词
组织工程脂肪
脂肪间充质干细胞
碱性成纤维细胞生长因子
Adipose tissue engineering
Mesenchymal stern cells derived from adipose tissue
Basic fibroblast growth factor (bFGF)