摘要
【研究目的】报道了一种快速简便地提取高质量植物总DNA的改良CTAB法。【结果】应用该法能简便快速地去除多糖等干扰物质,从而获得高质量的木犀科苦丁茶总DNA。所得DNA长度接近21kb,OD260/OD280在1.8左右。【结论】以此DNA为模板获得带条清晰、重复性高的ISSR-PCR扩增结果,且在一定范围内模板用量对ISSR-PCR扩增结果基本无影响。
A rapid and simple CTAB method for plant genomic DNA with high quality was presented. By this method the most of polyhexose could be fast and simply removed, and high quality genomic DNA from the Kudingcha species in oleaceae could be obtained. The molecular weight of isolated DNA was 21 kb nearly, and the OD260/OD280 of DNA was about 1.8. Using the DNA for template in ISSR-PCR, the amplification patterns were distinct and high reproducible, and the consistency of DNA template was basically not effected on the amplifying results of ISSR-PCR over a certain range.
出处
《中国农学通报》
CSCD
2008年第3期44-47,共4页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目"冬青属苦丁茶种质资源的遗传差异及其分类地位的研究"(30060040)
贵州省自然科学基金资助项目"西南地区木犀科主要苦丁茶分子标记"(黔科合 J 字[2005]2033 号)
