IL-2和IL-3基因共转染白血病细胞的体内外生物学特性

In vitro and in vivo biological characteristics of IL 2 and IL 3 genes cotransfected leukemia cells

目的：研究白细胞介素２（ＩＬ－２）、白细胞介素３（ＩＬ－３）基因共转染瘤苗的体内外生物学特性。方法：用ＩＬ－２重组腺病毒（Ａｄ－ＩＬ－２）、ＩＬ－３重组腺病毒（Ａｄ－ＩＬ－３）转染ＦＢＬ－３红白血病细胞株，观察其体外增殖能力及体内致瘤性的变化。结果：ＩＬ－２、ＩＬ－３共转染的ＦＢＬ－３细胞１周内能较稳定地分泌ＩＬ－２和ＩＬ－３，体外增殖能力无明显变化，但体内致瘤性明显下降，ＩＬ－２、ＩＬ－３共转染的ＦＢＬ－３体内致瘤性下降最为显著，肿瘤生长明显延缓，存活期显著延长。结论：ＩＬ－２，ＩＬ－３基因的转入导致ＦＢＬ－３细胞体内致瘤性的下降可能是由于激发体内免疫功能所致。

Objective:To study the growth characteristics of IL 2 and IL 3 genes co transfected FBL 3 cells. Methods: The FBL 3 cells were transfected with Ad IL 2 and /or Ad IL 3, in vitro proliferation and in vivo tumorigenicity were observed. Results: High levels of secreted IL 2 and IL 3 could remain for one week after transfection, and the transfected leukeimia cells became unchanged in vitro growth and decreased tumorigenicity. The tumorigenicity decreased most significantly when FBI 3 cells were transfected with both IL 2 gene and IL 3 gene. The tumor growth was significantly delayed and survival time was significantly prolonged. Conclusion: The inhibition of tumor growth is most likely dependent on IL 2 and IL 3 induced immune response in vivo.