亚低温对大鼠脑缺血损伤的保护作用

Protective effect of mild to moderate hypothermia on cerebral ischemia in rats

目的：验证亚低温对脑缺血损伤的保护作用。方法：２４只ＳＤ大鼠随机分为对照组（ｎ＝７），缺血组（ｎ＝９），亚低温组（ｎ＝８），参照Ｐｕｌｓｉｎｅｌｉ和Ｂｒｉｅｒｌｅｙ的方法建立脑缺血动物模型，观察Ｎａ＋－Ｋ＋－ＡＴＰ酶活性、脑组织含水量和丙二醛（ＭＤＡ）含量的改变。结果：与对照组和缺血组相比，亚低温组能阻止Ｎａ＋－Ｋ＋－ＡＴＰ酶活性的降低（Ｐ＜０．０１）、降低脑组织含水量（Ｐ＜０．０５）和ＭＤＡ含量（Ｐ＜０．０１）。结论：亚低温具有保护血脑屏障，减轻脑水肿，减少Ｃａ２＋内流，抑制氧自由基的产生等作用。

Objective: To evaluate the protective effects of mild to moderate hypothermia on cerebral ischemia. Methods: Twenty four SD rats were randomly divided into control group (n=7), ischemic group (n=9) and hypothermic group (n= 8) by means of Pulsinelli and Brierley’s animal model. The brain water content, malonaldehyde content and activity of Na + K + ATPase were observed. Results: Compared with the control and ischemic group, mild to moderate hypothermia could reduce brain water content (P<0.05) and malonaldehyde content (P<0.01), while increase the activity of Na + K + ATPase (P<0.01). Conclusion: These data show that mild to moderate hypothermia may play an important role in protecting blood brain barrier, alleviating brain edema, reducing influx of calcium and inhibiting oxygenic free radical production.