同源四倍体青花菜雄配子体的发育过程

Development Process of Male Gametophyte in Autotetraploid Broccoli

利用"爱氏苏木精染色—冬青油透明"技术,观察研究了同源四倍体青花菜(2n=4x=36)雄配子体的动态发育过程。结果表明:同源四倍体青花菜与相应的二倍体在雄配子的整个发育过程上表现相似,细胞核经一次有丝分裂产生营养核与生殖核,生殖核再经一次有丝分裂形成两个精核,最终形成3-细胞成熟花粉,具4个或3个萌发孔;雄配子体发育过程中异常频率为12.14%,败育主要发生在单核期。

This paper first reported the dynamic development of male gametophyte in autotetraploid broccoli using Ehrlich＇s hematoxylin staining-methyl salicylate clearing technique. Results showed that the male gametophyte in autotetraploid broccoli shared the similar development with that in diploid broccoli, which could give rise to one vegetative cell and one generative cell by one miotic division, while the generative cell could divide into two sperms by the other miotic division and the mature pollen was 3-celled with four or three apertures. Abnormality appeared mainly in uninuclete stage of male gametophyte development with an average frequency of 12. 14%.