摘要
在离体培养条件下,比较了秋水仙素不同浓度、不同处理时间诱导彩色马蹄莲体细胞染色体加倍的效果。结果表明,0.05%秋水仙素处理24h的诱变效果最佳,诱变率高达52%。用组织培养结合不定芽诱导技术获得了稳定的多倍体。对变异株进行细胞学观察后发现,二倍体对照2n=2x=32,变异株2n=4x=64,为四倍体,另外还发现六倍体细胞的存在。
Coloured Zantedeschia aethiopia was treated with colchicines at different concentration and time duration in vitro. The results showed： the best effect was obtained by the treatment of 0. 05 % colchicines with 24 hours, and its induction rate was 52%. Chromosome observation found that the chromosome number of treated plants was 64, and the chromosome number of the control was 32, indicating the tetraploid （2n = 4x = 64） of coloured Zantedeschia aethiopica is induced successfully.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第3期443-446,共4页
Acta Horticulturae Sinica
基金
国家科技部项目(2005DKA21006)
关键词
马蹄莲
多倍体
秋水仙素
染色体
Zantedeschia aethiopica
polyploid
colchicines
chromosome
