p21 ras、p53、c-myc、PCNA在子宫内膜腺癌中的表达

Expression of p21 ras, p53, c myc, and PCNA in endometral adenocarcinoma

目的：探讨癌基因与子宫内膜腺癌的关系。方法：应用免疫组化ＬＳＡＢ法对３３例原发性子宫内膜腺癌进行ｐ２１ｒａｓ，ｐ５３，ｃ－ｍｙｃ和ＰＣＮＡ的检测。结果：ｐ５３表达为７８．８％，ｐ２１ｒａｓ表达为５１．５％，ｃ－ｍｙｃ表达为８１．８％，ＰＣＮＡ表达为９７％。均明显高于正常增殖期子宫内膜（Ｐ＜０．０５）。ｐ２１ｒａｓ和ｐ５３在正常子宫内膜无表达，因此比ｃ－ｍｙｃ在子宫内膜腺癌的表达更特异。本研究显示ｐ５３与子宫内膜腺癌的病理分级有关（Ｐ＜０．０５），ｐ２１ｒａｓ与转移有关（Ｐ＜０．０５），在子宫内膜腺癌中，ｐ５３的表达与ｐ２１ｒａｓ的表达之间呈显著相关（Ｐ＜０．０１），本研究显示３种癌基因（ｐ２１ｒａｓ，ｐ５３和ｃ－ｍｙｃ）共同表达占４８．５％。结论：ｐ５３和ｐ２１ｒａｓ共同与子宫内膜腺癌的发生和发展有关。子宫内膜腺癌是多种基因作用的结果。

Purpose To explore the relation between oncogene and endometral adenocarcinoma; Methods The expressions of p21 ras, p53, c myc and PCNA were studied in 33 case of endometral adenocarcinoma by LSAB immunohistochemical technique; Results The results showed that the positivity rates of p53, pzlras, c myc and PCNA were 78 8%, 51 5%,81 8% and 97% respectively. The expressions of p21 ras, p53, c myc and PCNA in endometral adenocarcinoma were significantly higher than those in normal endometrum ( P< 0 05). The p21 ras expression was associated with metastasis ( P< 0 05). The p53 expression was correlated with histological grade ( P< 0 05). There was significant correlation between the expression of p53 and that of p21 ras in endometral adenocarcinoma ( P< 0 01). The coexpressions of p21 ras, p53 and c myc were 48 5%; Conclusions p53 cooperates with p21 ras in the pathogenisis and development of endometral adenocarcinoma. Endometral adenocarcinoma is the result of the cooperation of many oncogenes.