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蓝狐与银狐褪黑激素受体1A基因的克隆及序列分析 被引量：1

Cloning and Sequence Analysis of Melatonin Receptor 1A from Arctic Fox and Silver Fox
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摘要 根据其它物种褪黑激素受体（melatonin receptor,MTNR）1A基因核酸序列的同源性设计引物,PCR扩增蓝狐和银狐MTNR1A基因的546 bp DNA序列,GenBank登录号分别为EU170442和EU170443。蓝狐和银狐MTNR1A基因DNA序列同源性为99.1%,编码的氨基酸同源性为99.4%,与人、鼠及绵羊MTNR1A基因同源性为84%-86%,与鸡MTNR1A基因同源性为74%。 A pair of primers were designed to amplify the sequences of melatonin receptor 1A from arctic fox and silver fox according to the sequence homology of MTNR1A from other animal species. PCR product was separated, purified and cloned with the pUCm-T vector. The cloned sequences were 546 bp in length（GenBank Accession No. EU170442 and EU170443）. The homology of the nucleotide sequence and amino acid sequence was 99.1% and 99.4% between arctic fox and silver fox, respectively. They had 84% to 86% homology to MTNR1A sequences of human, mouse and sheep, and 74% to MTNR1A sequence of chicken.
作者 闫守庆 王慧芳 祝万菊
机构地区 吉林大学畜牧兽医学院
出处 《中国畜牧兽医》 CAS 2008年第3期24-26,共3页 China Animal Husbandry & Veterinary Medicine
关键词 蓝狐 银狐 褪黑激素受体1A基因 序列分析 arctic fox silver fox melatonin receptor 1A sequence analysis
分类号 Q785 [生物学—分子生物学]
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参考文献9

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中国畜牧兽医
2008年 第3期

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