期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

The Function Identification of the Candidate Disease-Resistance Gene in Cabbage

The Function Identification of the Candidate Disease-Resistance Gene in Cabbage
下载PDF
导出
摘要 In order to understand the function of TuR2, a candidate disease-resistance gene was isolated from cabbage, we transformed it into mustard （Brassicajuncea L. Linshicaoyaozi） which was susceptible to TuMV through Agrobacterium tumefacine-mediated method. Transgenic plants were detected by Southern blotting and Northern blotting. Our results confirmed that the TuR2 gene had been integrated into the mustard genome, and it showed different expression levels among primary transplants （T0）. The primary transplants （T0） and the first progenies of transgenic plants （T1） were inoculated with TuMV in a greenhouse. The transgenic plants had high TuMV-resistance, whereas the serious virus disease symptom was observed in CK （no transformation plants）. The TuR2 gene in the first progenies of transgenic plants （T1） showed dominant monogenic inheritance. Compared with CK, the progenies containing TuR2 gene had stronger resistance to TuMV. The TuR2 gene which was isolated from cabbage had the function of TuMV-resistance. In order to understand the function of TuR2, a candidate disease-resistance gene was isolated from cabbage, we transformed it into mustard （Brassicajuncea L. Linshicaoyaozi） which was susceptible to TuMV through Agrobacterium tumefacine-mediated method. Transgenic plants were detected by Southern blotting and Northern blotting. Our results confirmed that the TuR2 gene had been integrated into the mustard genome, and it showed different expression levels among primary transplants （T0）. The primary transplants （T0） and the first progenies of transgenic plants （T1） were inoculated with TuMV in a greenhouse. The transgenic plants had high TuMV-resistance, whereas the serious virus disease symptom was observed in CK （no transformation plants）. The TuR2 gene in the first progenies of transgenic plants （T1） showed dominant monogenic inheritance. Compared with CK, the progenies containing TuR2 gene had stronger resistance to TuMV. The TuR2 gene which was isolated from cabbage had the function of TuMV-resistance.
作者 CAO Bi-hao LEI Jian-jun XIANG Chen-bin CHEN Guo-ju ZENG Guo-ping David J. Oliver ZHAO Shuang LU Ling-ling
机构地区 Horticulture Biotechnology Research Institute Department of Botany 不详
出处 《Agricultural Sciences in China》 CAS CSCD 2008年第3期314-320,共7页 中国农业科学（英文版）
基金 supported by the National Natural Science Foundation of China(30270912) Postdoctoral Foundation of China(2003033410).
关键词 TuR2 gene function identification TuMV-resistance TuR2 gene, function identification, TuMV-resistance
分类号 S634 [农业科学—蔬菜学]
  • 相关文献

参考文献11

  • 1曹必好,宋洪元,雷建军,宋明,杨朝辉.结球甘蓝抗TuMV相关基因的克隆[J].Acta Genetica Sinica,2002,29(7):646-652. 被引量：18
  • 2Bent A F,Kunkel B N,Dahlbeck D,Brown K L,Schmidt R,Giraudat J,Leung J,Staskawicz B J.RPs2 of Arabidopsis thaliana:a leucine-rich repeat class of plant disease-resistance gene[].Science.1994
  • 3Cai D,,Kleine M,Kifle S,Haeioff H J.Positional cloning of a gene for nematode resistance in sugar beet[].Science.1997
  • 4Farnham G,Baulcombe D C.Baulcombe Artificial evolution extends the spectrum of viruses that are targeted by a disease- resistance gene from potato[].Proceedings of the National Academy of Sciences of the United States of America.2006
  • 5Johal G S,Briggs S P.Reductivity encoded by the Hml disease-resistance gene in maize[].Science.1992
  • 6Latha A M,Rao K V,Reddy V D.Production of transgenic plants resistant to leaf blast disease in finger millet[Eleusine coracana (L.) Gaertn.][].Plant Science.2005
  • 7Sambrook J,Fritsch E F,Mamiatis T.Molecular Cloning[].A Laboratory Manual.1989
  • 8Whitham S,Dinesh-Kumar S P,Choi D,Hehl R,Corr C,Baker B.The product of the tobacco mosaic virus resistance gene N:similarity to Toll and the interleukin-1 receptor[].Cell.1994
  • 9Xiao S,Ellwood S,Calis O,Patrick E,Li T,Coleman M,Turner J G.Broad-spectrum mildew resistance in Arabidopsis mediated by RPW8[].Science.2001
  • 10Yoshimura S,Yamanouchi U,Katayose Y,Toki S,Wang Z X,Kono I,Kurata N,Yano M,Iwata N,Sasaki T.Expression of Xa1,a bacterial blight-resistance gene in rice, is induced by bacterial inoculation[].Proceedings of the National Academy of Sciences of the United States of America.1998

二级参考文献20

  • 1[1]Johl G S, Briggs S P. Reductase activity encoded by the HM1 disease resistance gene in maize. Science, 1992, 258:985～987.
  • 2[2]Jones D A. Thomas C M, Hammond-Kosack K E et al. Isolation of the tomato cf-9 gene for resistance to cladosporium fulvum by transposon tagging. Science, 1994, 266:789～793.
  • 3[3]Martin G B, Brommonsch cnkel S H, Chunwongse et al. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science, 1993, 262:1432～1436.
  • 4[4]Whtham S D, Dinesh-kumar S P, Choi D et al. The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor. Cell, 1994, 78:1101～1115.
  • 5[5]Lawrence G J, Finnegan E J, Ayliffe M A et al. The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. Plant Cell, 1995, 7:1195～1206.
  • 6[6]Bent A F, Kunkel B N, Dahlbeck D et al. RPS2 of Arabidopsos theliana: A leucine-rich repeat class of plant disease resistance gene. Science, 1994, 265:1856～1860.
  • 7[7]Mindrinos M, Katagiri F, Yu G L et al. The Arabidopsos thealiana disease resistance gene RPS2 encodes a protein containing a nucleotide binding site and leucine-rich repeat. Cell, 1994, 78:1089～1099.
  • 8[8]Song W Y, Wang G L, Chen L L et al. A receptor kinase-like protein encoded by the rice disease resistance gene Xa21. Science, 1995., 270:1804～1806.
  • 9[9]Cai D G, Michael K. Positions cloning og a gene for nematode resistance in sugar beet. Science., 1997., 275(7):832～834.
  • 10[10]Catherine F, Gabriele S, Beat K. Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of weat. The Plant Joural, 1997, 11(1):45～52.

共引文献17

Agricultural Sciences in China
2008年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部