河南大尾寒羊染色体核型与G-带分析

Ananlysis on the karyotype and G-banding of chromosomes in He′nan large Tail Sheep

【目的】研究河南大尾寒羊染色体核型和G-带。【方法】分离培养河南大尾寒羊外周血淋巴细胞,制备染色体标本和G-带标本,分析河南大尾寒羊的染色体核型和G-带。【结果】河南大尾寒羊二倍体染色体数目为2n=54,公羊核型54,XY;母羊核型54,XX。27对染色体中包括26对常染色体和1对性染色体,常染色体中有3对为中着丝粒染色体,23对为端着丝粒染色体;性染色体中,X染色体为最大的端着丝粒染色体,Y染色体为最小的中着丝粒染色体。河南大尾寒羊G-带与其他绵羊品种的G-带基本一致。【结论】河南大尾寒羊的染色体数目为:2n=54,公羊核型54,XY;母羊核型54,XX。河南大尾寒羊G-带与其他绵羊品种的G-带基本一致。

【Objective】 The research is to study the karyotype and G-banding pattern of the chromosomes in Henan large tail sheep.【Method】 The chromosome samples and G-banding samples were made by the way of the peripheral blood lymphocyte culture to analyze the karyotype and G-banding pattern of the chromosomes in Henan large tail sheep.【Result】 The number of the chromosomes in Henan large tail sheep is 2n=54,the karyotypes of male and female sheep are 54,XY and 54,XX,respectively.There are 26 pairs of autosomes and 1 pair of sex-chromosome.Among 26 pairs of autosomes,3 pairs are metacentric chromosomes,23 pairs are telocentric chromosomes.X chromosome is the biggest subtelocentric chromosome and Y chromosome is the smallest submetacentric chromosome.The analysis of G-banding patterns for macro-chromosome of them indicates no remarkable differences between the Henan large tail sheep and sheep of other breeds.【Conclusion】 The number of the chromosomes in Henan large tail sheep is 2n=54,the karyotypes of male and female sheep are 54,XY and 54,XX respectively.The G-banding patterns of the He′nan large tail sheep are consistent with these of other sheep breeds.