摘要
【目的】研究山杏种皮黑色素与Cu2+和Zn2+络合的相关性质,为山杏种皮黑色素以及其他植物黑色素的开发利用提供依据。【方法】采用电位滴定、紫外-可见光差示吸收光谱和红外光谱对山杏种皮黑色素与Cu2+和Zn2+的络合物进行分析。【结果】Cu2+和Zn2+与山杏种皮黑色素络合后,均能促进山杏种皮黑色素酸性位点的去质子化作用,但Cu2+的促进作用更强,结合更为牢固;随pH值升高,山杏种皮黑色素中解离出的络合位点增多,络合Cu2+能力增强。【结论】Cu2+和Zn2+主要与山杏种皮黑色素中羰基发生络合作用,氨基和羟基参与了络合。
【Objective】 The coordination chemistry of Cu2+ and Zn2+ in the melanin from testae of Prunus armeniaca var.ansu was investigated to lay a foundation for utilization of the melanin and other phytomelanins.【Method】 Potentiometric titration,ultraviolet-visible (UV-Vis) differential and Fourier transform infrared(FT-IR) spectroscopy were employed.【Result】 The complexation of the metal ions promote deprotonation of the acidic melanin sites and Cu2+ complexation more obviously and more tightly than that of Zn2+.The melanin had more binding capacity with Cu2+ at high pH value than at neutral condition.【Conclusion】 The essential interactions of the metal ions(Cu2+ or Zn2+) went on with carboxyl groups and amine and hydroxyl were also involved in complexation formation.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2008年第3期197-202,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家林业局“948”引进项目(2004-4-52)
关键词
山杏种皮
黑色素
金属结合
电位滴定
红外光谱
teatae Prunus armeniaca var.ansu
melanin
metal-binding
potentiometric titration
infrared analysis