烧伤后白细胞与内皮细胞粘附分子介导作用的研究

The role of PMN CD 11b /CD 18 on the increasing PMN adhesion to endothelial cells induced by severe burn injury

为研究白细胞粘附依赖于细胞粘附分子的介导作用，作者通过离体实验观察分析了烧伤血清对外周血中性粒细胞（ＰＭＮ）ＣＤ１１ｂ／ＣＤ１８表达的影响及ＣＤ１１ｂ／ＣＤ１８在烧伤后ＰＭＮ粘附中的介导作用。结果表明：（１）烧伤血清使ＰＭＮＣＤ１１ｂ／ＣＤ１８分子表达和ＰＭＮ与肺微血管内皮细胞（ＰＭＥＣ）的粘附率增高。（２）ＣＤ１１ｂ／ＣＤ１８单抗不仅能使正常ＰＭＮ与ＰＭＥＣ的粘附率下降５０％，也使烧伤血清激活的ＰＭＮ与ＰＭＥＣ粘附率下降至烧伤血清激活前水平。我们认为，严重烧伤能使外周血ＰＭＮＣＤ１１ｂ／ＣＤ１８表达明显增高，ＣＤ１１ｂ／ＣＤ１８不但介导基础状态下的ＰＭＮ粘附，更是介导烧伤后引起的大量ＰＭＮ与ＰＭＥＣ粘附的主要分子。

The process of leukocyte adhesion was mediated by the intercellular adhesion molecular.In this study,both the influence of burn serum on the expression of PMN CD 11b /CD 18 and the role of CD 11b /CD 18 on the burn serum stimulated PMN adhesion to endothelial cells(EC) were investigated. The expression of CD 11b /CD 18 on PMN incubated with burn serum for 2 hours was increased obviously. So was PMN adhesion to EC. Monoclone antibody to PMN CD 11b /CD 18 could reduce the normal PMN adhesion to EC by half and block the increasing PMN adhesion induced by burn serum. These indicated that PMN CD 11b /CD 18 may be the main adhesion molecular participating in the increasing PMN adhesion to EC due to severe burn.