摘要
以华盖木嫁接植株当年生枝为外植体,以1/2MS为基本培养基,添加不同浓度的6-BA、KT、NAA进行3因素3水平正交试验,筛选诱导华盖木腋芽萌发的适宜培养基。结果表明,培养基的激素组成和浓度对外植体的萌发率和褐化率都有极显著影响,1/2 MS+6-BA 1.0mg/L+KT 0.5mg/L是筛选出的诱导腋芽萌发的适宜培养基,萌芽率为53.33%,褐化率为13.33%。
Take the tissue culture with the 1 year- old branch of Manglietiastrum sinicum as explant. And take 1/2 MS as the culture medium, with different concentration of 6 -BA, KT, NAA for L9 （34） perpendicularity experiment. The results showed that the component and concentration of hormone of the culture medium had extreme marked effect on the germination and browning of the explants. The suitable culture medium for the induction of the germination of the axillary buds was 1/2 MS ＋ 6 - BA 1.0 mg/L ＋ KT 0.5 mg/L. The germination rate is 53.33% , and the browning rate is 13.33%
出处
《林业科技》
北大核心
2008年第2期1-3,共3页
Forestry Science & Technology
基金
四川农业大学都江堰分校科技基金项目"华盖木生物学特性及繁育技术研究"
项目编号N200702
四川省科技厅应用基础研究项目"红色警报级几乎绝迹种华盖木人工繁育技术体系研究"
关键词
华盖木
组织培养
萌芽率
褐化率
Manglietiastrum sinicum
Tissue culture
Germination rate
Browning rate
