千里光总黄酮的抗炎作用研究

Study of the Antiinflammatory Effect of Total Flavonoids from Senecio scandens Buch-Ham

目的探讨千里光总黄酮的抗炎作用。方法通过二甲苯致小鼠耳廓肿胀实验、腹腔注射醋酸致小鼠腹腔毛细血管通透性增高实验以及小鼠棉球肉芽肿实验,观察千里光总黄酮的抗炎作用;制作小鼠气囊滑膜炎模型,测定炎症渗出物中白细胞数(WBC)和前列腺素E2(PGE2)含量。结果千里光总黄酮对二甲苯致小鼠耳廓肿胀、醋酸致小鼠毛细血管通透性的增加以及小鼠棉球肉芽肿的形成均有明显的抑制作用;千里光总黄酮高中低剂量组炎症渗出液中白细胞数和PGE2含量明显低于空白对照组。结论千里光总黄酮对多种炎症模型均有明显的对抗作用,为千里光抗炎作用的主要有效部位之一,且此作用部分是与炎症因子PGE2的产生和释放受抑制有关。

Objective To investigate the anti - inflammatory effect of the total flavonoids in Senecio scandens Buch - Ham. Methods Various inflammatory models,including the swelling of ear induced by xylene in mice, the permeability increase of blood capillary by acetic acid in mice, and the cotton - pellet granuloma of the mice were used to explore the anti - infammation of the total flavonoids. Air sac models of synovitis in mice were set up and the amount of white blood cell （WBC）and the content of prostaglandin E2 （ PGE2 ） were measured in inflammatory exudan. Results The total flavonoids had significant inhibitory action on auris swell induced by xylene in mice and penetration of capillary vessel in mice. The general flavonoids also decreased the proliferation of granuloma caused by implantation cotton pellet in mice significantly. The amount of WBC and content of PGE2 in the experimental groups were obviously lower than those in the control groups. Conclusion The total flavonoids has significant anti -inflammation, which is relevant to the inhibition of the production and release of the inflammatory factor PGE2.