枳陈汤对Wistar大鼠胃排空影响的实验研究

Effects of Zhichen Tang on Gastric Emptying in Wistar Rats

目的观察枳陈汤对Wistar大鼠胃排空的作用,探讨其可能的作用机制。方法将Wistar大鼠随机分成A组(枳陈汤组)和B组(正常对照组)。用药5周后,用同位素法检测其胃液体排空功能,用微量羟胺法检测其红细胞乙酰胆碱酯酶活性,用放免法检测其血浆胃动素。结果A组和B组的30 min胃排空率分别为(43.35±11.56)%和(31.66±6.81)%(P<0.05)。A组的红细胞乙酰胆碱酯酶活性和血浆胃动素分别为(0.418±0.066)μmol/30 min和(125.81±25.58)pg/ml,B组为(0.291±0.056)μmol/30 min和(91.18±25.19)pg/ml。A组均比B组的高(P<0.05)。结论枳陈汤可有效地促进大鼠胃排空功能,其机制可能与通过增强胆碱能神经功能和提高血浆胃动素水平有关。

Objective To observe the effect of the herbal medicine Zhichen Tang on the gastric emptying in wistar rats and to investigate its mechanism. Methods Wistar rats were randomly divided into two groups -group A（ Zhiehen Tang） group B （control）. After treating with Zhiehen Tang for 5 weeks, 30 - min gastric liquid empyting rate, erythroeyte aeetyleholinesterase activity and plasma motilin content were measured by radioistopie teehniology, mieroeolorimetrie and radioim munoassay, respectively. Results The experiment indicated that 30 min gastric emptying rates of group A and B were （43.35 ± 11.56）% and （31.66± 6.81 ）% ,respectively（ P 〈0.05）. The erythrocyte aeetyleholinesterase activity and the plasma motilin content of group A were also significantly higher than those of group B. Conclusion Zhichen Tang can promote liquid gastric emptying by improving the cholinergie nerve function as well as increasing the plasma motilin content,