摘要
目的建立测定薄荷通吸入剂主要成分含量的气相色谱-质谱联用方法(GC.Ms法)。方法以环已酮、萘为双内标物,采用气相色谱-质谱-选择离子检测(GC—MS/SIM)联用方法中对薄荷通吸入剂主要成分进行含量测定。SPB.1701(30m×0.25mm×0.25μm)石英毛细管柱;80~250℃程序升温,选择m/z43,95,71,120离子碎片分别对桉油精、樟脑、薄荷脑和水杨酸甲酯含量进行检测。结果桉油精、樟脑、薄荷脑、水杨酸甲酯线性范围分别为0.24~97μg/ml(r=1,0000)、5.00~202μg/ml(r=0,9999)、2.30~947μg/ml(r=0.9998)、0.68—271μg/ml(r=1.0000);检测限分别为2.4,5.0,2.3,6.8ng/ml;平均回收率为98%~101%,相对标准偏差为1.9%~2.5%。结论该方法快速、准确、灵敏度高、重现性好,可用于同类产品质量控制。
Objective A new method for the determination of main compositions in Bo He Tong Xi Ru Ji by Gas Chromatography - Mass Spectrometry with selected ion monitor (GC-MS/SIM) with Naphthalene and Cyclohexanone as internal standard was developed. Methods SPB-1701 column and temperature program: from 80℃ to 250℃. The conditions of GC -MS were optimized and the selected ions were m/z 43,95,71 and 120, respectively. Results The linear range of Eucalyptol, Eucalyptol, Camphor, Methyl Salicylate were 0.24 -97,5.00 - 202,2.30 - 947,0.68 - 271 μg/ml. The detection limit of them were 2.4,5.0,2.3, 6.8 ng/ml. The recovery range was 98% - 101% and the RSD was less than 2.5%. Conclusion The method can be successfully applied to the determination of Main Compositions in Bo He Tong Xi Ru Ji.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第3期678-679,共2页
Lishizhen Medicine and Materia Medica Research
关键词
气相色谱-质谱
选择离子检测
薄荷通吸入剂
Gas Chromatography - Mass Spectrometry
Selection monitor
Main compositions
Bo He Tong Xi Ru Ji