气相色谱-质谱-选择离子检测联用法测定薄荷通吸入剂主要成分的含量

Determination of Main Compositions in Bo He Tong Xi Ru Ji by GC-MS/SIM

目的建立测定薄荷通吸入剂主要成分含量的气相色谱-质谱联用方法（GC．Ms法）。方法以环已酮、萘为双内标物，采用气相色谱-质谱-选择离子检测（GC—MS／SIM）联用方法中对薄荷通吸入剂主要成分进行含量测定。SPB．1701（30m×0．25mm×0．25μm）石英毛细管柱；80～250℃程序升温，选择m／z43，95，71，120离子碎片分别对桉油精、樟脑、薄荷脑和水杨酸甲酯含量进行检测。结果桉油精、樟脑、薄荷脑、水杨酸甲酯线性范围分别为0．24～97μg／ml（r=1，0000）、5．00～202μg／ml（r=0，9999）、2．30～947μg／ml（r=0．9998）、0．68—271μg／ml（r=1．0000）；检测限分别为2．4，5．0，2．3，6．8ng／ml；平均回收率为98％～101％，相对标准偏差为1．9％～2．5％。结论该方法快速、准确、灵敏度高、重现性好，可用于同类产品质量控制。

Objective A new method for the determination of main compositions in Bo He Tong Xi Ru Ji by Gas Chromatography - Mass Spectrometry with selected ion monitor （GC-MS/SIM） with Naphthalene and Cyclohexanone as internal standard was developed. Methods SPB-1701 column and temperature program： from 80℃ to 250℃. The conditions of GC -MS were optimized and the selected ions were m/z 43,95,71 and 120, respectively. Results The linear range of Eucalyptol, Eucalyptol, Camphor, Methyl Salicylate were 0.24 -97,5.00 - 202,2.30 - 947,0.68 - 271 μg/ml. The detection limit of them were 2.4,5.0,2.3, 6.8 ng/ml. The recovery range was 98% - 101% and the RSD was less than 2.5%. Conclusion The method can be successfully applied to the determination of Main Compositions in Bo He Tong Xi Ru Ji.