紫外分光光度法优选黄芩总黄酮的提取工艺研究

Study on the Extraction Optimization of Flavonoids from Scutellaria baicalensis by UV-spectrophotometry

目的探讨黄芩中总黄酮提取工艺中的提取溶剂用量、提取时间、提取次数对黄芩中总黄酮得率的影响。方法采用紫外分光光度法,测定不同提取溶剂用量、不同提取时间、不同提取次数所得到总黄酮含量。结果通过正交实验,找到黄芩中总黄酮提取的最佳提取工艺条件为:30%乙醇提取3次,剂量12,10,10倍,时间2,1,1 h。结论该研究可以为工厂化黄芩总黄酮的生产过程制定合理的提取工艺提供理论依据,对生产具有一定的指导意义。

Objective To study the effects of factors like solvent dosage, extraction time and extraction times on the production extraction of flavonoids from Scutellaria baicalensis. Methods To determine the content of the total flavonoids by UV - spectrophotometry. Results A better method of extraction of flavonoids from Scutellaria baicalensis was found through orthogonal test,the influences of factors like solvent dosage, extraction time and extraction times on total flavones were analyzed. Results showed the best conditions of extraction were 30% ethanol 3 times, dosage 12,10,10 times,time 2,1,1 hours. Conclusion Theoretical foundation of the reasonable procedure can be provided for the flavonoid production from Scutellaria baicalensis in the factory and it is significant for the production.