反相高效液相色谱法测定酸枣仁颗粒中酸枣仁皂苷A与酸枣仁皂苷B的含量

RP-HPLC Dertermination of Contents of Jujubaside A and Jujubaside B in Ziziphus jujuba Mill(ZJM) Granules

目的 建立酸枣仁颗粒中酸枣仁皂苷A与酸枣仁皂苷B的含量测定方法。方法反相高效液相色谱法，采用Hy．persilC18柱为分析柱，使用以乙腈和水为流动相的二元梯度洗脱方法，检测波长为201nm，流速为1ml·min^-1。结果酸枣仁皂苷A在50．01—198．2μg·ml^-1与峰面积呈良好的线性关系（r=0，9998，n=5），平均回收率为99．41％；酸枣仁皂苷B在66．25—209．0μg·ml^-1与峰面积呈良好的线性关系（r=0．9998，n=5），平均回收率为99．34％。结论该法简便、快速、重现性好，适用于酸枣仁颗粒中酸枣仁皂苷A与酸枣仁皂苷B的定量分析。

Objective To develop a method for the determination of Jujubaside A and Jujubaside B in Ziziphus jujuba Mill（ZJM） Granules. Methods An RP - HPLC method was set up with Hypersil 18 column. The mobile phase consisted of acetonitrile and water, and a gradient elution program was applied. The DAD detector was set at 201 nm. Results The calibration curve of Jujubaside A was linear in the range of 50.01 - 198.2μg · ml^ - 1, the average recovery was 99.41% ; the calibration curve of Jujubaside B was linear in the range of 66, 25 - 209, 0μg · ml^-1 ,the average recovery was 99.34%, Conclusion The method is appropriate for the determination of Jujubaside A and Jujubaside B.