大熊猫RPS15 cDNA的克隆及序列分析

cDNA Cloning and Sequences Analysis of RPS15 from Giant Panda

本研究运用RT-PCR技术,首次从大熊猫Ailuropoda melanoleuca的肌肉组织总RNA中成功克隆了核糖体蛋白S15(RPS15)基因的表达序列,并对其进行了初步分析。结果表明:大熊猫RPS15基因的表达序列全长为442bp,开放阅读框(ORF)为438bp,编码145个氨基酸,该蛋白的分子量为17.0401KDa,等电点为10.3,含有2个依赖于cAMP和cGMP的蛋白激酶磷酸化位点,5个蛋白激酶C磷酸化位点,4个N-酰基化位点及1个RPS19蛋白signature位点。进一步分析发现,大熊猫RPS15基因的表达序列及其编码的氨基酸序列与已报道的部分哺乳动物具有很高的相似性。

The expression sequence of ribosomal protein S15 （RPSI5） gene from muscle tissue of Ailuropoda melanoleuca was amplified successfully through RT-PCR technique for the first time, which was sequenced and analyzed preliminarily. The result showed that the full length of the expression sequence was 442 bp containing an ORF of 438 bp. The deduced protein sequence showed that the protein was couaposed of 145 amino acids and its estimated molecular weight was 17. 0401 kDa with a pl of 10, 39. There were 4 different patterns of functional sites ： two cAMP and cGMP-dependent kinase phosphorylation sites; one ribosomal protein S19 signature site; four N- myristoylation sites; five casein kinase C phosphorylation sites in the RPS15 protein. Further analysis indicated that the expression sequence of RPS15 and the protein encoded were highly homologous to some mammals reported.