酶法提取葡萄皮渣高活性膳食纤维工艺的研究

Production of high activity dietary fiber from grape pomace by enzymatic method

以葡萄皮渣为原料,经过低浓度NaOH预处理后,采用纤维素酶降解,从中得到水溶性膳食纤维含量较高的高活性膳食纤维。结果表明,NaOH最佳预处理浓度为0.5%;纤维素酶最佳作用条件为:酶用量50μL/g,作用温度50℃,pH7.0,时间6h,提取物中的总膳食纤维含量达到60.7%,其中水溶性膳食纤维占28.8%,是原料的2倍多,说明酶解法提取葡萄皮渣膳食纤维是可行的。

High activity dietary fiber with high content of soluble dietary fiber was extracted from grape pomace by cellulose enzyme after alkali pretreatment. The results showed that the optimal pretreatment concentration of NaOH was 0.5%. The optimal treatment conditions for cellulose enzyme were： dosage 50gL/g, hydrolysis temperature 50℃, pH7.0, hydrolysis time 6h. The total dietary fiber （TDF） in the extracts could reach 60.7% with soluble fiber accounting for 28.8%.