奶牛子宫组织内肥大细胞分布规律的研究

Regularity of Mast Cell Distribution in the Uterine Tissue of the Cow

取4头奶牛的新鲜子宫,经生理盐水冲洗后,分别于子宫角、子宫颈和子宫体部位取材,放入10%中性福尔马林固定液中固定,对全层子宫进行连续切片,用甲苯胺蓝染色,再用油镜观察子宫内膜层、肌层和外膜层的肥大细胞数量。经生物统计分析发现,奶牛子宫组织内肥大细胞主要分布在子宫内膜层,在子宫肌层和外膜层较少;奶牛子宫各部位的内膜层、肌层和外膜层的肥大细胞构成比相近。

The whole uterus from 4 cows were cleaned in 0.9% NaCl solution before sampling from the uterine horn, cervix and uterine body respectively. The samples were fixed in the 10% neutral formaldehyde solution, and a continuous slices of the whole uterine layer were stained by the method of toluidine blue stain. The mast cells in the uterine tissues were examined and counted under a light microscope. The results showed that the mast cells of the uterine tissues of cow distributed mainly in the mucous membrane. There was a less distribution of the mast cells in the tunica adventitia of the cow uterine tissues. The distribution of mast cells in different uterine layers of the uterine horn, cervix and uterine body was mutual independent. The above results indicated that the mast cells showed a regular pattern of distribution in the uterine tissue of the cow.