癫癎患者血清、脑脊液神经元特异性烯醇化酶的测定及意义

Serum and cerebrospinal fluid levels of neuron-specific enolase in epileptic patients

目的探讨癫癎患者癎性发作后对神经元和血脑屏障的损伤。方法采用酶联免疫法测定癫癎患者在癎性发作后2d内血清和脑脊液(CSF)中神经元特异性烯醇化酶(NSE)的含量,与非神经系统疾病的神经症对照组对比分析。结果患者组CSF中NSE较对照组升高,差异有统计学意义;但血清中NSE含量与对照组差异无统计学意义。结论癫癎患者癫癎发作后脑脊液中NSE升高,提示癎性发作对中枢神经的神经元有损伤;而血中NSE正常,提示血脑屏障正常。

Objective To explore the damage of neuron and blood-brain barrier in epilepteic patients（EP） after seizures. Methods The serum and cerebrospinal fluid（CSF） levels of neuron-specific enolase（NSE） in the epileptic patients were dynamical determined within two days after seizures by enzyme-linked immune sandwich assay. The serum and CSF levels of NSE in control group were tested too. Results The CSF levels of NSE in EP group within two days after seizures were significantly higher than those in control group （P〈0. 01 ）,while there were no sigificant differences in serum NSE levels between both groups. Conclusion NSE in CSF increases after seizures. It suggests that seizures could lead to neuronal damage. But NSE in serum has no change after seizures, it suggests that blood-brain barrier is normal.