肺神经内分泌癌FHIT蛋白表达及其临床病理意义的研究

Clinicopathological significance of expression of fragile histidine triad protein in neuroendocrine lung carcinoma

目的:探讨脆性三联组氨酸(FHIT)蛋白在肺神经内分泌癌中的表达及其临床病理意义。方法:采用二步法非生物素免疫组化技术检测90例肺神经内分泌癌组织中FHIT蛋白表达并分析其与各临床病理指标的关系。结果:FHIT蛋白表达定位于癌细胞质;并与组织学类型(P=0.006)、临床分期(P=0.003)、性别(P=0.031)、肿瘤大小(P=0.010)、淋巴结转移(P=0.000)以及预后(P=0.000)相关;与患者年龄(P=0.408)、大体类型无关,P=0.528;FHIT表达为肺神经内分泌癌的独立预后因子,P=0.000。结论:FHIT蛋白在肺神经内分泌癌的发生、演进过程中起着重要作用,并可作为分子指标检测患者预后。

OBJECTIVE：To investigate the expression of fragile histidine triad （FHIT） protein in neuroendocrine lung carcinoma and evaluate its clinicopatholigical significance. METHODS： The expression of FHIT protein was detected by immunohistochemistry in 90 cases of neuroendocrine lung carcinoma, and the relationship between the expression of FHIT protein and clinicopathological indices was analyzed statistically. RESULTS：The positive staining of FHIT protein was mainly localized in the cytoplasm of tumor cells, and the expression of FHIT protein was significantly corresponding to the histological type （ P = 0. 006 ）, clinical stage（P=0. 003）, sex（P = 0. 031）, lymph node metastasis （P = 0. 000） and prognosis（P= 0. 000）, but not to the age（P= 0. 408）and gross type, P= 0. 528. FHIT expression was an independent prognostic factor, P = 0. 000. CONCLUSION：Loss of FHIT protein may play an important role in the development and evolution of neuroendocrine lung carcinoma, and it may become the molecular marker to predict the prognosis of the patients.