摘要
目的:检测14-3-3γ在卵巢上皮性癌组织的表达,探讨在卵巢上皮性癌发病机理中14-3-3γ蛋白家族的作用机制。方法:应用实时荧光定量PCR方法检测14-3-3γ在15例正常卵巢组织和52例卵巢上皮性癌组织中的表达差异,应用原位杂交方法检测14-3-3γ mRNA在正常卵巢组织和卵巢上皮性癌组织中的定位。结果:实时荧光定量PCR结果经过Mann-Whitney U检验,表明卵巢癌组的14-3-3γ mRNA水平显著高于正常卵巢组。14-3-3γ mRNA定位于卵巢癌细胞中,在正常卵巢组织中没有明显信号出现。结论:在人上皮性卵巢癌中,14-3-3γ转录上调,14-3-3γ mRNA定位于癌细胞。由此推测,14-3-3γ可能参与了人上皮性卵巢癌的发生。
Objective：To explore the expression of 14-3-3γin primary epithelial ovarian cancers. Methods：The expression of 14-3-3γ gene was evaluated by real-time fluorogenetic quantitative PCR （RFQ-PCR） in 15 cases with normal ovarian surface epithelium and 52 cases with primary epithelial ovarian cancer. The location of 14-3-3γ in normal human ovarian tissues and epithelial ovarian cancer tissues were evaluated by in situ hybridization. Results：QRT-PCR showed that the average quantity of 14-3-3γ mRNA in epithelial ovarian cancer tissues was significantly higher than that in normal human ovarian tissues by Mann-Whitney U test （P 〈 0. 05, P =0. 0003 ）. The in situ hybridization showed that 14-3-3γ mRNA existed in cytoplasm of ovarian cancer cells,There were no signaling in normal ovarian tissues. Conclusion ：14-3-3γ is overexpressed in ovarian cancer tissues on transcriptional level, so 14-3-3γ may play an important role in the pathogenesis of epithelial ovarian cancer.
出处
《现代妇产科进展》
CSCD
北大核心
2008年第2期99-101,共3页
Progress in Obstetrics and Gynecology
基金
黑龙江省教育厅科学技术研究基金资助项目(No:10541113)
