PHBV膜与PHBV/SGBG复合材料联合促进骨再生

Ossification in Bone Defects after Treatment with PHBV Membrane and PHBV/SGBG Composite

【目的】探讨聚羟基丁酸/羟基戊酸共聚酯(PHBV)膜与3-羟基丁酸-co-3-羟基戊酸共聚物/溶胶-凝胶生物活性玻璃(PHBV/SGBG)多孔复合材料促进骨再生的能力。【方法】实验共采用8只健康杂种犬,每只犬分别于左、右胫骨各制作2个直径为5mm的穿通骨髓腔的圆洞形骨缺损区,间隔2cm。双侧胫骨共建立4个骨缺损模型,将其随机分为四组:A组,骨缺损内填充柱状PHBV/SGBG材料,并在其上方覆盖PHBV膜;B组,骨缺损上方覆盖PHBV膜;C组,骨缺损内填充柱状PHBV/SGBG材料;D组,不放置任何东西。分别于术后2、4、8、12周各处死2只动物,组织学观察不同实验条件下骨缺损区新骨组织生长情况。【结果】所有植入材料组(A组、B组、C组)均未出现植入物排异反应。其中A组术后2周时,已有明显的新生骨岛形成;术后12周骨缺损区新生成熟骨组织再生。与其它实验组相比,A组的骨缺损修复最早,新生骨的质量最好,骨结构与周围正常骨基本一致。而空白对照组中,骨痂修复中可见纤维组织形成,骨缺损区修复不全。【结论】PHBV膜覆盖技术和PHBV/SGBG复合材料植入技术结合,具有促进骨缺损区的骨组织修复的协同作用;PHBV膜和PHBV/SGBG复合材料有望成为应用于引导骨组织再生的新一代材料。

[Objective] To evaluate the efficiency of polyhydroxybutyrate-hydroxyvalerate （PHBV） membrane and 3-bydroxybutyrate-co-3-bydroxyvalerate/sol-gel bioactive glass （PHBV/SGBG） composite for promoting bone regeneration in vivo. [Methods ] Eight healthy dogs were used as experimental animals. Bone defects with 5 mm in diameter through marrow were created bilaterally in the tibia of the dogs respectively. Four identical defects in one dog were separated into four groups： group A （treatment with PHBV membrane and PHBV/SGBG composites）, group B （treatment with PHBV membrane alone）, group C （treatment with PHBV/SGBG composites alone）, and group D （empty control）. Autos-control was carried out in this study. Two dogs were sacrificed in 2, 4, 8, and 12 weeks after operation, respectively. The repairing effect was assessed by histological observation for the regeneration of osseous tissue. [ Results ] The clearance of the implants could not be seen in all experimental groups （group A, B, and C） during all implant periods. There were obviously new bone islands in group A at the 2nd week after the implantation, and there was new mature bone regeneration at the 12th week. The bone rehabilitation in group A was the earliest and the best among all the groups, and the new bone architecture was exactly the same as the natural osseous tissue around the defect. While, in group D, the fibrous tissue was observed inside the osteotylus, and the defect achieved insufficient repair.[ Conclusion] PHBV membrane produces a synergistic effect to enhance new bone formation in the defect when combined with PHBV/SGBG composite together, these two novel materials may become promising membrane and composite for guided bone regeneration.