赤魟组织粗提物的抗肿瘤作用

Anti-tumor Effect of Rude Extracts of Dasyatis akajei

采用四甲基偶氮唑盐(MTT)微量酶反应比色法检查赤魟三种组织(皮肤、软组织和软骨)粗提物对体外培养的人癌细胞生长的影响,用腹腔注射或灌胃法检查赤魟三种组织粗提物对小鼠移植性肉瘤180(S180)和肝癌(H22)生长的影响。结果表明,赤魟组织粗提物对体外培养的人早幼粒白血病细胞(HL-60)、人子宫颈癌细胞(HeLa)和人低分化鼻咽癌细胞(CNE-2Z)的生长无明显影响,赤魟组织粗提物无论腹腔注射还是灌胃均显著抑制小鼠肉瘤和小鼠肝癌的生长,且对体重无明显影响;当三种组织粗提物以500mg/kg/d(相当生药量,下同)的剂量连续腹腔注射(ip)15d时,对S180的抑制率分别为63.9%、47.2%和80.3%;以3500mg/kg/d连续灌胃(ig)15d时,对S180的抑制率分别为66.9%、61.5%和66.9%;以500mg/kg/d连续腹腔注射15d时,对H22的抑制率分别为43.3%、43.3%和80.4%。

The present study was undertaken to investigate a possible anti-tumor effect of the rude extracts from skin, soft tissue and cartilage of Dasyatis akajei. MTT [3-（4,5-dimethylthiazol-z-yl）-2,5-diphe- nyl-tetrazolium bromide] assay was performed to measure the cytotoxicity of three sorts of rude extracts to HL-60 cells, HeLa cells and CNE-2Z cells, and their in vivo anti-tumor effects were investigated by measuring the tumor weight of tumor-bearing mice （S180 and H22）. The three sorts of rude extracts did not show obvious effects on the growth of HL-60 cells, HeLa cells and CNE-2Z cells. However, when administered 500 mg/kg/d （to be equal the quantity of crude drug, ip）or 3 500 mg/kg/d （ig） for 15 d, they caused 63.9%, 47.2% and 80.3%, and 66.9%, 61.5% and 66.9% inhibition rates on S180, respectively; when administered 500 mg/kg/d （ip） for 15 d, they caused 43.3%, 43.3% and 80.4% inhibition rates on H22, respectively. In conclusion, the three sorts of rude extracts did not show obvious effects on the growth of HL-60 cells, HeLa cells and CNE-2Z cells, but they showed marked anti-tumor effect in vivo.