TCDD对斑马鱼脂质过氧化作用的初步研究

Studies on Lipid Peroxidation of TCDD in Zebrafish

将150条斑马鱼随机等分为5组:2,3,7,8-四氯代二苯并二噁英(TCDD)4个染毒组(染毒剂量分别为0.1μg/L、0.2μg/L、0.4μg/L和0.8μg/L)和空白对照组,水质接触染毒5d后,采用分光光度法测定其肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)的活力,研究TCDD对斑马鱼的脂质过氧化作用。结果发现,TCDD各剂量染毒组MDA含量均有所增加,其中0.2μg/L组差异显著(P<0.05),0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组SOD活性均有所下降,其中0.2μg/L、0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组GST活力均降低且差异极显著(P<0.01)。一定剂量的TCDD能引起MDA含量增加,使SOD和GST活力降低,对斑马鱼具有脂质过氧化作用。

To study the lipid peroxidation of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin （TCDD） in zebrafish, 150 zebrafish were randomly and equally divided into 5 groups： four test groups were exposed to TCDD at 0.1 μg/L, 0.2 μg/L, 0.4 μg/L, 0.8 μg/L , respectively in addition to one control group. Five days after the animals were exposed to the polluted water with different doses of TCDD, respectively, the level of malondialdehyde （MDA） and the activities of superoxide dismutase （SOD） and glutathionetransferase （GST） in liver of zebrafish were determined by means of spectrophotometry. It was found that the MDA level showed some increasing tendency in which 0.2 μg/L of TCDD exposure group was different （P〈0.05） and 0.4μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from the control group （P〈0.01）, that SOD activity showed decreasing tendency and 0.2 μg/L, 0.4 μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from control group （P〈0.01）, and that GST activity in all TCDD treated groups showed significantly difference from the control group （P〈0.01）. It wasconcluded that TCDD at certain doses caused hepatic lipid peroxidation in zebrafish by induction of MDA increase and SOD and GST inactivation.