通阳散结方对球囊损伤大鼠血管内膜转化生长因子-β1的影响

Effect of composite prescription of activating Yang and removing obstruction(通阳散结方) on transforming growth factor-β1 of vascular endothelium in rats with saccule injury

目的探讨通阳散结法及其组方射心通胶囊对血管球囊损伤实验大鼠术后血管内膜增生的干预作用。方法将30只SD大鼠随机分为假手术组、球囊损伤模型组和中药复方干预组,每组10只。连续给药并饲养8周,各组于6周末行颈动脉内膜剥脱术,分离出颈外动脉,结扎其远端,在近端作一切口,逆行插入球囊导管,造成颈动脉内膜损伤;假手术组只分离出颈外动脉,结扎其远端,未行球囊损伤。术后继续喂养2周后处死各组实验大鼠,取颈动脉损伤血管段,标本经相应处理后观察其形态学变化及转化生长因子-β1(TGFβ-1)免疫组化和逆转录-聚合酶链反应(RT-PCR)结果。结果①模型组动脉内膜增生较假手术组明显,而中药复方干预后内膜增生面积明显减少,差异均有统计学意义(P均<0.01);②模型组TGF-β1的蛋白及mRNA表达较假手术组明显增加,而中药复方干预组能明显减少模型动物TGF-1β的蛋白及mRNA表达,差异有统计学意义(P均<0.01)。结论通阳散结法及其组方可通过抑制TGF-β1的表达,阻止新生内膜的形成,从而干预球囊损伤术后的血管重构。

Objective To approach the intervention effect of activating Yang and removing obstruction composite prescription（通阳散结方）and its capsule of Shexintong（射心通）on rats with hyperplasia of vascular endothelium after vascular saccule injury test. Methods Thirty Spregue-Dawley （SD） rats were randomly divided into 3 groups： sham operation group, saccule injury group, Chinese composite prescription group （each n= 10）. The medicine was given and they were fed for 8 weeks, at the end of the 6th week the exfoliation of carotid artery endothelium was performed： the external carotid artery was separated, its distal end was ligated, at its proximal end, a cut was made, a saccule tube was reversely inserted, causing the carotid artery tunica intima injury; in the sham operation group, the external carotid artery was isolated, its distal end was ligated, but no saccule injury was made. After operation, the rats were continuously fed for two weeks, then they were sacrificed, and the damage part of the carotid artery was taken out. The pathomorphologic changes were investigated and the transforming growth factor-β1（TGF-β1） of the specimen was observed by immunohistological method and reverse transcription-polymerase chain reaction（RT-PCR）. Results ①The hyperplasia of the artery endothelium was more obvious in the saccule injury or the model group than that in the sham operation group （P〈0.01）. After the intervention of the composite prescription, the endothelial area of hyperplasia was smaller significantly than that of the model group （P〈0. 01）. ② The expressions of TGF-β1 protein and TGF-β1 mRNA increased a lot in the model group compared with those in the sham operation group （P〈0.01）; in the intervention of Chinese composite prescription group, the expressions of TGF-β1 protein and TGF-β1 mRNA were obviously decreased compared with the model group （P〈0.01）. Conclusion Composite prescription of activating Yang and removing obstruction can inhibit the expression of TGF-β1, prevent the proliferation of endothelial cells, thus, it may intervene the reconstruction of blood vessel after saccule injury.