第一复叶期喷施农达41%水剂对大豆生理指标的影响

Impact of Roundup 41% aqua on physiological index of soybean at one-trifoliolate leaf stage

采用随机区组设计方法,在第一复叶期喷施不同剂量的农达41%水剂,研究了其对不同大豆品种叶片叶绿素含量指数、莽草酸含量和SOD活性等生理指标的影响。结果表明:喷施农达41%水剂可以抑制抗草甘膦大豆"RR1"、"RR2"和普通大豆"晋大75"、"晋豆27"叶片的叶绿素合成,"RR1"、"RR2"在最高剂量(1.85kg.hm-2)时受抑制不明显,而"晋大75"和"晋豆27"在较低剂量(0.46kg.hm-2)时即明显受害。"RR1"和"RR2"在高剂量(1.85kg.hm-2)农达41%水剂下,莽草酸仍积累较少,而"晋大75"和"晋豆27"在较低剂量(0.46kg.hm-2)时体内莽草酸就大量积累。随农达41%水剂剂量增加,"RR1"和"RR2"体内的SOD活性增加,"晋大75"和"晋豆27"在较低剂量0.46kg.hm-2时诱发了体内SOD活性,但随着剂量的增加,体内SOD活性受到抑制。总的来说,抗草甘膦大豆对农达41%水剂的抗性大于普通大豆,"RR1"的抗性大于"RR2","晋豆27"(晚熟)大于"晋大75"(早熟)。

Random complete block design was used to research the impact of spraying Roundup 41% aqua on chlorophyll content index, shikimic acid content and SOD activity of glyphosate-resistant soybean （＂ RR1 ＂, ＂ RR2＂） and ordinary soybean （ ＂Jinda75＂, ＂Jindou27＂ ） cuhivars at one-trifoliolate leaf stage. The results indicate that Roundup 41% aqua decreases chlorophyll content index of the 4 soybean cultivars. The degree of decrease in ＂RR1＂ and ＂RR2＂, which are not obviously inhibited by the highest Roundup dosage （ 1.85 kg. hm^-2 ） , is smaller than that in ＂Jinda75＂ and ＂Jindou27＂, which are significantly inhibited by lower Roundup dosage （0.46 kg. hm^-2 ）. RR1 and ＂RR2＂ accumulate less shikimie acid at higher Roundup dosage （ 1.85 kg. hm^-2 ）, while ＂Jinda75＂ and ＂Jindou27＂ begin accumulating shikimie acid even at a lower dosage （0.46 kg. hm^-2）. Roundup 41% aqua prompts SOD activities in ＂RR1 ＂ and ＂RR2＂ from the initial stage, while that of ＂Jinda75＂ and ＂Jindou27＂ is only prompted at lower dosage （0.46kg.hm^-2） and at the initial growth period. In summary therefore, the order of glyphosate-resistance in the 4 soybean cuhivars is： ＂RR1＂ 〉 ＂ RR2＂ 〉 ＂ Jindou 27 ＂ （ latter maturity） 〉 ＂Jinda 75 ＂ （ early maturity）.