外周血单个核细胞HBV的复制状态与干扰素-γ表达和分泌的关系

Relationship of HBV replication with expression and secretion of IFN-γ in PBMC

目的:研究HBV侵犯外周血单个核细胞(PBMC)及对IFN-γ表达和分泌的影响.方法:慢性HBV感染者60例,巢式PCR检测PBMC中HBV共价闭合环状DNA(cccD-NA);ELISA法检测血清IFN-γ水平;实时定量RT-PCR方法检测PBMC中IFN-γ mRNA的表达水平.结果:48.3%(29/60)的患者PBMC中检测到cccDNA;血清IFN-γ水平低于对照组(P<0.05);PBMC中IFN-γ mRNA表达水平低于对照组(P<0.01);PBMC中cccDNA阳性组IFN-γ表达和分泌水平低于cccDNA阴性组(P<0.01).结论:HBV慢性感染者PB-MC中IFN-γ表达和分泌水平降低.

AIM： To study how HBV affect the expression and secretion of IFN-γ in PBMC. METHODS： sitty patients with chronic hepatitis B were studied. HBV cccDNA in PBMC were amplified by nest-PCR. The level of IFN-γ in the serum was determined by ELISA. The expression of IFN-γ mRNA in PBMC was determined by real - time RT-PCR. RESULTS ： HBV cccDNA in PBMC were determined by 48.3% （29/60） in patients. The serum level of IFN-γ in the patients was lower than that in control group（ P 〈0.05）. The expression of IFN-γ in PBMC wsa lower in the patients than in control group （ P 〈 0.01 ）. The expression and secretion of IFN-γ were lower in cccDNA positive group than in cccDNA negative group （ P 〈 0.01 ）. CONCLUSION： The expression and secretion of IFN-γ in patients with chronic hepatitis B are decreased.