不同黑麦染色体对普通小麦主要贮藏蛋白组成的影响

The Effect of Different Rye Chromosomes on the Expression of Storage Protein in Common Wheat

为了解不同遗传背景的黑麦染色体对普通小麦主要高分子量谷蛋白亚基(HMW-GS)和醇溶蛋白组成的影响,采用SDS-PAGE和A-PAGE分析了两套小麦-黑麦(Holdfast-King和中国春-Impire)双二倍体和不同附加系的HMW-GS和醇溶蛋白组成。结果表明:(1)两份双二倍体的HMW-GS都表达出小麦亲本带型和一条迁移率与双亲不一致的条带,醇溶蛋白呈共显性表达。(2)Holdfast-King的1R附加系HMW-GS带型与其双二倍体带型一致,醇溶蛋白呈共显性表达;3R附加系的HMW-GS缺失受体亲本条带并表达出一条迁移率与双亲有差异的条带,其醇溶蛋白缺失受体亲本条带;其余附加系(2R,4R^7R)的HMW-GS和醇溶蛋白带型与其受体亲本带型一致。(3)中国春-Impire的1R附加系HMW-GS带型与其双二倍体一致,醇溶蛋白只表现受体小麦亲本带型,2R到7R附加系的HMW-GS和醇溶蛋白带型都与小麦亲本带型一致。这表明小麦-黑麦异源双二倍体中小麦和黑麦的基因组会相互影响,引起籽粒贮藏蛋白组成表达上的差异;同一种黑麦的不同染色体对籽粒贮藏蛋白组成表达的影响也有差异。

In order to determine the effect of different rye chromosomes on the expression of storage protein in common wheat,the high-molecular-weight glutenin subunits （HMW subunits of glutenin in bread wheat and HMW secalins in rye） composition and the polymorphic gliadin forms of 7 Holdfast-ing, Chinese Spring-Impire wheat-rye alien addition lines and 2 wheat-rye amphidiploids were sepa- rated by SDS-PAGE and A-PAGE. The results showed： （1） The HMW prolamin in 2 amphidiploids all presented the same banding pattern to wheat parent. In addition, amphidiploids presented a band with different mobility to parents. The gliadin forms in amphidiploids showed codominance. （2） There were three patterns of HMW prolamin identified in Holdfast-King addition lines. 1R addition line showed the same HMW prolamin pattern to amphidipoid, as well as its gliadin form. 2R, 4R, 5R, 6R, 7R addition lines all showed the same HMW prolamin to recipient parent which contain 5 bands while 3R addition lines had only 4 bands and a band had different mobility from amphidiploid and the other addition lines. Similarily, 2R, 4R, 5R, 6R and 7R addition lines had the same gliadin form to recipient parent, but 3R disappeared 2 bands in ω-region. （3） The banding patterns of HMW prolamin in 1R addition line of Chinese Spring-Impire overlapped those of its parents. But its gliadin form expressed the banding pattern of recipient parent as well as the rest addition lines （from 2R to 7R）. The wheat genome and the rye genome should take effect on each other in amphidiploid, which could have influence on the expression of genes controlling the seed storage protein. And different rye chromosomes from the same rye specie might take different effect on the seed storage protein expression.