中温螺旋藻新品系的选育研究

STUDIES ON MIDDLE TEMPERATURE STRAINS SELECTION OF SPIRULINA PLATENSIS

利用超声波处理钝顶螺旋藻出发品系（Sp）NS－90020，获得单细胞螺旋藻；然后用NTG诱变筛选获得突变品系即SpALT_1；比较出发品系和突变品系的一般形态和生理生化特征，出发品系和突变品系的一般形态有较大差异。与出发品系相比，突变品系藻丝体显著变短，螺旋数目大大减少。它们光合作用温度曲线的差异性表明，突变品系Sp－ALT_1的最适放氧温度比出发品系低。用4℃低温处理这两个品系，突变品系比出发品系更能忍受低温。以上表明，Sp－ALT_1是一株较能忍受低温的中温品系。此外Sp－ALT_1的蛋白质含量比出发品系高9．5％。

Single cells of SPirulina platensis were made by treating the initial strain(Sp)NS-90020 with Ultrasonic Homogenizer, then the mutant, SP-ALT, were selected afterinducing these single cells with NTG. And their general morphology,physiological features and biochemical composition were studied comparatively. There are some morphological differences between the initiall strain and the mutant. The filaments of the mutant show much shorter lengh and fewer numbers of the helix than of the initial strain.The temperature curves of photosynthesis showed that the optimum temperature of oxygen-evolution of SP-ALT, was lower than that of the initial strain. When they weretreated at 4'C,the mutant could endure much lower temperature than the initial strain.These showed that SP-ALT, was a middle temperature strain. In addition,the protein inSP-ALT, increased by 9. 5% compared with the initial strain.