15-Mt-PGF_(2α)对 CCL_4 所致大鼠离体肝细胞损伤的保护作用

PROTECTIVE EFFECTS OF 15 METHYL PROSTAGLANDIN F 2α ON PRIMARY CULTURED RAT HEPATOCYTE AGAINST CCl 4 INDUCED INJURY

采用大鼠离体肝细胞原代培养２４ｈ，并利用四氯化碳ＣＣｌ４造成急性肝细胞损伤模型，检定１５－甲基－前列腺素Ｆ２α（１５－Ｍｔ－ＰＧＦ２α）对肝细胞损伤的影响。结果表明：（１）１５－Ｍｔ－ＰＧＦ２α可显著降低中毒肝细胞脂质过氧化物水平，抑制肝细胞脂质过氧化，并降低谷丙转氨酶（ＧＰＴ）和谷草转氨酶（ＧＯＴ）水平，稳定脂质膜。（２）显著促进中毒肝细胞ＲＮＡ和ＤＮＡ的合成。（３）超微结构证实１５－Ｍｔ－ＰＧＦ２α能减轻ＣＣｌ４对肝细胞脂质膜，染色质，线粒体，内质网和核蛋白体的损害。

The effect of 15 Mt PGF 2α on CCl 4 induced injury of primary cultured hepatocytes was studied. 15 Mt PGF 2α treatment (2mg/L) significantly decreased CCl 4 (10mmol/L) induced damages of primary cultured rat heptocytes as indicated by decreases GPT and GOT leakage and LPO production. 15 Mt PGF 2α significantly promoted 3 H uridine incorporatin into RNA and thymidine incorporation into DNA of the rat hepatocytes. Cytopathology study showed that 15 Mt PGF 2α attenuated damages of mitochondria, endoplasmic reticulum and ribosome caused by CCl 4. 15 Mt PGF 2α appeared to maintain the stability of rat hepatocytes by inhibiting lipid peroxidation. These results indicated that 15 Mt PGF 2α has notable protective effect on primary cultured rat hepatocytes against CCl 4 induced damage by reducing lipid peroxidation and promoting synthesis of RNA and DNA.