大肠癌患者P53基因的突变研究

MUTATION STUDY OF P53 GENE IN PATIENTS WITH LARGE BOWEL CARCINOMA

本研究应用聚合酶链反应——单链构象多态ＰＣＲ—ＳＳＣＰ银染技术对２８例大肠癌患者的癌组织Ｐ５３基因第５，６，７和８外显子进行检测，发现有１５例存在基因突变，突变率为５４％。这一结果表明Ｐ５３基因突变在大肠癌中的普遍存在，并在大肠癌的发生过程中起着重要作用；同时表明本研究所采用的ＰＣＲ—ＳＳＣＰ银染技术具有简便、快速、灵敏和安全的特点，可应用于肿瘤的临床基因诊断。

Using PCR-SSCP silver staining technique,28 patients with large bowel carcinoma were analysed for mutation in exons 5,6,7,and 8 of p53 gene.15 cases of mutations were founded.The mutation rate was 54%.It was showed that p53 gene mutation is a common event in large bowel carcinoma,which plays an important role in the progression of large bowel carcinoma.This is a simple,sensitive,rapid and reliable method.It is useful in clinical gene diagnosis for large bowel carcinoma.