培养人淋巴细胞微核测试法检测非整倍体诱变剂的研究

A MICRONUCLEUS ASSAY WITH CULTURED HUMANLYMPHOCYTES IN VITRO FOR DETECTION OF ANEUGENIC EFFECTS

直接用培养人淋巴细胞微核测试系统，采取控制化学品处理培养细胞和恢复时间，对已知非整倍体诱变剂长春新碱（ＶＢＬ）进行微核检测，实验结果显示，在设定的微核检测程序中，阴性对照和断裂剂丝裂霉素Ｃ（ＭＭＣ）未诱发微核率（ＭＮＦ）的显著增加（Ｐ＞０．０５）而ＶＢＬ却引起ＭＮＦ显著增加（Ｐ＜０．０１）。结果揭示培养人外周血淋巴细胞微核测试法可能成为一种新的人类化学品非整倍体诱变剂的检测方法。

In order to look for a simpler method for detection of chemical aneugens,we have evaluated an improved micronucleus assay in cultured human lymphocytes,controlling culture times after treatment of chemicals and recovery times.The results showed that clastogen Mitomycin C(MMC) did not result in micronucleus(MN) increase.Whereas,the known aneugen Vinblastine(VBL) induced a marked increase in MN(P<0.01) in the experimental schedule designed.Our study suggested that the MN assay in cultured human lymphocytes established in our experiment could become an improved method for detection of chemical aneugens.