HPLC测定盆炎康颗粒中芍药苷的含量

Determination of Peoniflorin in Penyankang Granules by RP-HPLC

目的:建立测定盆炎康颗粒中芍药苷含量的RP-HPLC方法。方法:采用反相高效液相色谱法,Kromasil C_(18)柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水溶液(15:85),流速为1.0mL·min^(-1),检测波长为230nm,柱温为27℃。结果:芍药苷在0.2～1μg与峰面积呈良好的线性关系(r=0.999 3),加样回收率在99%～101%,平均回收率为101.18%,RSD=1.23%(n=5)。结论:本方法简便、准确,重现性好,适用于盆炎康颗粒中芍药苷含量的测定。

Objective： To establish an RP-HPLC method for determination of the content of peoniflorin in penyankang granules. Methods： A Kromasil C18 column （250mm × 4.6mm, 5μm）with a solvent system consisting of acetonitrile-water（15：85） was used with a flow rate of 1.0 mL·min^-1 and the detecting wavelength was 230nm. The column temperature was27℃. Results ： The peoniflorin showed good linear correlation within0.2-1 μg （ r = 0. 999 3 ）, The average recovery of peoniflofin was 101.18 % with RSD of 1.23 % （n = 5 ）. Conclusion： The method is simple and can be used to determine peoniflorin with satisfactory accuracy and reproducibility.