当归补血汤对大鼠永久性局灶性脑缺血后脑源性促红细胞生成素的影响

Effect of Danggui Buxue Tang on Erythropoietin in Rats with Permanent Focal Cerebral Ischemia

目的:观察当归补血汤对大鼠永久性局灶性脑缺血的保护作用及对脑源性促红细胞生成素(erythropoie-tin,EPO)表达的影响。方法:将动物随机分为假手术组、模型组和给药组,采用改良线栓法建立大鼠永久性局灶性脑缺血模型,按缺血时间每组分为4h、1d和7d亚组,给药组灌胃当归补血汤。在缺血相应时间后取材,行甲苯胺蓝染色,观察组织病理变化;行TTC染色,计算脑梗死体积百分比;行Fluoro-Jade B(FJB)染色,计算半暗带阳性细胞数;行EPO免疫荧光染色,计算半暗带阳性细胞数。结果:与模型组相比,当归补血汤可以减轻缺血脑组织的病理改变,降低缺血7d亚组脑梗死体积百分比(P<0.05),减少缺血1d、7d亚组半暗带FJB阳性细胞数(P<0.05,P<0.01);给药组缺血1d、7d亚组半暗带EPO阳性细胞数明显多于模型组(P<0.01)。结论:当归补血汤对永久性局灶性脑缺血大鼠具有一定的保护作用,可能与增强脑缺血后脑源性EPO表达有关。

Objective： To observe the protective effect of Danggui Buxue Tang （DGBXT） on ischemia brain via brain rooted erythropoietin. Methods： The permanent focal cerebral ischemia model was established by middle cerebral artery occlusion （MCAO）. 45 SD rats were enrolled randomly into three groups, sham group, model group, DGBUDgroup; According to the occlussion time, each group was further divided into 4h, ld and 7d subgroups. Toluidine blue stain to observe the pathologic change; TTC stain and then calculate the percentage of infarction volume; Fluoro-Jade B（FJB）stain to mark degenerated cells and count the FJB positive cells in the penumbra area; Immunofluorescence stain to detect EPO, count the EPO positive cells in the penumbra area. Resuits： Contrasted to the model group, DGBXI） can reduce the infraction volume in 7d subgroup （P〈0. 05） and the FJB positive cells in the penumbra area in ld and 7d subgroup（P〈0. 05, P〈0. 01）. The EPO expression in the penumbra area of DGBXD groups are significantly higher than that of model group in ld and 7d subgroup（P〈0. 01）. Conclusion： DGBXD can protect the ischemia brain, via enhancing brain rooted EPO.