PARP抑制与小鼠结肠癌CT26细胞生长活性的关系

Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines

目的:探讨PARP抑制与结肠癌CT26细胞生长活性的关系。方法:采用PARP抑制剂5-氨基异喹啉酮(5-aminoisoquinolinone,5-AIQ)处理小鼠结肠癌CT26细胞,MTT法检测CT26细胞离体生长,免疫荧光双标、激光共聚焦检测结肠癌CT26细胞核内PARP与NF-κB p65的表达。结果:不同浓度的5-AIQ处理组(0.1mmol/L、0.5mmol/L、1.0mmol/L)结肠癌CT26细胞生长抑制率分别为17.52%、27.63%和39.93%,抑制率随5-AIQ浓度的增加而增加,差异具显著性(P<0.05)。PARP与NF-κB p65在CT26细胞核内存在共表达,且呈正相关(P<0.05)。5-AIQ处理组与5-AIQ未处理组相比,PARP与NF-κB p65的表达均减弱,差异具显著性(P<0.05),PARP与NF-κB p65亦呈正相关(P<0.05)。结论:PARP抑制剂5-AIQ可以抑制结肠癌CT26细胞生长活性。其可能与PARP抑制,使NF-κB活性降低有关。PARP在结肠癌生长过程中可能具有重要作用。

Objective：To investigate the relationship between poly （ADP-ribose）polymerase （PARP） inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro. Methods：The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro. M3W assay was used to determine the growth activity of CT26 cells. The expressions of PARP and NF-κ B p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope. Results：The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52% ,27.63% and 39.93%, respectively. The inhibitory rate increased with the raising of the 5-AIQ concentration （0.1mmol/L,0.5mmol/L,and 1.0mmol/L）,and the difference was significant（P〈0.05）. There was a co-expression and positive correlation of PARP and NF-κ Bp65 in the nuclei of colon carcinoma CT26 cells（P〈0.05）. The expressions of PARP and NF-κ B p65 were attenuated in 5-AIQ-treated CT26 cell groups, and the correlation of PARP and NF-κ B p65 was also positive（P〈0.05）. Conclusion：PARP inhibitior 5-AIQ can inhibit the growth activity of colon carcinoma CT26 cells in vitro, and this may be through inhibiting PARP to reduce the activity of NF-κ B. PARP may play an important role in the proliferation of colon carcinoma.