脂质体转染survivin siRNA对A549细胞增殖及凋亡的影响

Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell

目的:探讨脂质体转染survivin siRNA对人肺腺癌A549细胞增殖、凋亡的影响。方法:人工合成抑制survivin基因的siRNA片段,通过脂质体转染到A549细胞内,倒置荧光显微镜下观察细胞形态学变化;用四氮唑盐(MTT)法观察转染后24h,48h,72h对细胞增生的影响;流式细胞术检测各组细胞周期变化和凋亡指数;RT-PCR检测survivin mRNA的表达。结果:转染siRNA后的A549细胞,细胞增殖受到显著抑制,细胞明显出现凋亡,survivin基因mRNA表达显著下降。结论:应用RNA干扰靶向抑制survivin基因可以显著抑制A549细胞增殖,促进细胞凋亡。survivin可能成为肺癌基因治疗的一个新靶点。

Objective：To explore the effect of liposomal transfection of survivin siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines A549. Method：Survivin siRNA was synthetized and transfected into A549 cell by liposome. Cell morphological change was observed with inverted fluorescent microscope,cell proliferation was examined by MTT assay at 24h 48h and 72h after transfection, cell proliferation and apoptotic index detected by flow cytometry and survivin mRNA expression determined by RT-PCR. Results：For the A549 cell transfected with siRNA cell proliferation was ihibited significantly,cell apoptosis appeared obvious and the expression of survivin mRNA significantly decreased. Conclusion：Survivin siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines A549 and induce the cell apoptosis. Survivin might become a new target for lung carcinoma gene therapy.