大鼠窒息死后脑组织中iNOS的表达规律研究

Expression of iNOS on brain tissue in rats model of asphyxia death

目的:对比大鼠窒息死与其它原因死亡脑组织中iNOS的表达规律及法医学意义。方法:制作大鼠机械性窒息死亡、失血性休克死亡、颅脑损伤死亡及断颈死亡模型;应用免疫组织化学方法及图像分析技术检测大鼠死后iNOS在各死亡组不同时间段、各组脑组织中的表达情况。结果:iNOS在大鼠机械性窒息死亡,失血性休克死亡、颅脑损伤死亡的脑组织中都有表达,断颈死组无表达;机械性窒息死亡组iNOS表达明显高于其它各组,各组0h的表达都稍低于6h、12h、24h;且各组6h、12h、24h的表达趋于同一水平。结论:这一发现,为在死亡后24h内检测脑组织中iNOS表达可以作为推断窒息死的1个生物学指标。

Objective：To compare expression of iNOS on brain tissue in rats model of asphyxia death with other death groups and forensic significance. Methods：The production method of mechanical asphyxia death,hemorrhagic shock death,brain traumatic death and breaks the neck to execute group model;By means of immunohi stochemistry and image analysis technology to determinate the dead rats after each group at different times of iNOS in the brain tissue of expression. Results：iNOS in mechanical asphyxia death,hemorrhagic shock death and brain traumatic death have expressed,breaks the neck to execute was no expression and mechanical asphyxia death group was significantly higher levels than in all levels of other group,each group of Oh expression all slightly bottom to 6h, 12h,24h,Also each group of 6h, 12h,24h expression tends to the identical level o Conclusion：This discovery,in the 24 hours after the death of brain tissue iNOS expression can be inferred from the asphyxial death of a biological indicators