芝麻素对代谢综合征大鼠血糖、血脂及主动脉血管细胞黏附分子-1蛋白表达的影响

Effects of sesamin on blood glucose,blood lipids and vascular cell adhesion molecule-1 protein expression of aorta in rats with metabolic syndrome

目的:探讨芝麻素对代谢综合征大鼠血糖、血脂及主动脉血管细胞黏附分子-1(VCAM-1)蛋白表达的影响。方法:高脂高糖诱导大鼠代谢综合征24周,于诱导第9周连续口服芝麻素(120、60、30mg.kg-1.d-1)16周后,称大鼠体重、腹部脂肪重量,测血脂、血糖、血清总抗氧化能力和血清过氧化氢含量。HE染色观察主动脉病理变化,免疫组化法观察主动脉VCAM-1蛋白表达。结果:芝麻素120、60mg/kg组体重与腹部脂肪重量明显低于模型组(P<0.01),TG、TC、低密度脂蛋白胆固醇(LDL-C)和血糖水平明显降低(P<0.01),高密度脂蛋白胆固醇(HDL-C)水平明显升高(P<0.01);主动脉VCAM-1蛋白表达明显减少,病理损伤明显减轻;血清与血管总抗氧化能力明显提高,过氧化氢含量明显减少。结论:芝麻素可以降低代谢综合征大鼠血糖、血脂及主动脉VCAM-1表达,减轻血管病理损伤,具有防治动脉粥样硬化的作用。

AIM： To explore the effects of sesamin on blood glucose, blood lipids and vascular cell adhesion molecule-1 （VCAM-1） protein expression of aorta in rats with metabolic syndrome. METHODS： A highfat, refined-carbohydrate diet was given to rats to induce metabolic syndrome for 24 weeks. Sesamin （ 120, 60, 30 mg· kg^-1 ·d^-1） were given to the metabolic syndrome rats at the ninth week by intragastric administration, which were lasted for 16 weeks. Then, the body weight and abdominal fat of all rats were weighed and the levels of blood lipid, blood glucose, the total anti-oxidation capacity （T-AOC） and the hydrogen peroxide concentration in the serum were determined. In addition, the pathological change and the VCAM-1 protein expression of aorta were observed by H-E staining and immunohistochemical method respectively. RESULTS： Compared with those in model group, the body weight and abdominal fat of sesamin （ 120, 60 mg· kg^-1· d^-1 ） groups were obviously decreased, the levels of serum TG, TC, LDL-C and blood glucose were markedly decreased and the level of HDL-C was increased. The VCAM-1 protein expression of aorta was depressed. The pathological change was improved; The T-AOC and hydrogen peroxide concentrations of serum and aorta were decreased. CONCLUSION： Sesamin can obviously deserease the levels of blood lipid, blood glucose and depress the VCAM-1 protein expression of aorta, improve the pathological change of aorta and protect against the progression of atheroselerosis.