利用基因芯片检测水稻细菌性条斑病抗性相关基因

Detection of Genes Related to Resistance to Bacterial Leaf Streak in Rice Using Microarray

细菌性条斑病(简称细条病)是水稻的主要病害之一。为了鉴定细条病的抗性基因,我们利用Affymetrix的基因芯片对抗病品种Acc8558和感病品种H359中受细条病菌侵染调控的基因进行高通量的检测。在两品种中共检测到956个差异表达基因,其中12个基因在两品种中一致上调,54个基因在两品种中一致下调,20个基因在两品种中表达变化方向相反。对差异表达基因的基因本性、代谢路径和启动子进行了分析。比较发现有30个差异表达基因的位置与已报道的控制水稻细条病的QTL吻合。本研究为水稻细条病抗性基因的克隆提供了线索,为揭示水稻细条病抗性的分子遗传机理奠定了基础。

Bacterial leaf steak （BLS） is one of the most important diseases in rice. To identify BLS-resistant genes in rice, high-throughput detection of genes responding to the infection of BLS pathogen in a high-resistant variety Acc8558 and a high-susceptive variety I-I359 was performed using Affymetrix microrarray. A total of 956 differentially expressed genes （DEGs） were identified in the two varieties, of which 12 genes were consistently up-regulated and 54 consistently down-regulated in the two varieties, while 20 genes showed opposite expression change directions in the two varieties. Gene ontologies, pathways and promoters of the DEGs were analyzed. Comparison showed that 30 DEGs matched the positions of QTLs conferring resistance to BLS reported before. The results of this study will facilitate positional cloning of BLS-resistant genes and revealing the molecular genetic mechanisms of BLS resistance in rice.