^(125)Ⅰ-抗Tg-McAb与甲状腺癌细胞结合能力的影响因素研究

Effect of Some Factors on Binding of ^(125)Ⅰ-anti-Tg-McAb to thyroid Carcinoma Cells

模仿体内环境及放射免疫显像临床应用中遇到的实际问题，观察Tg浓度、pH值及碘化钾（KI）、青霉素和地塞米松等药物对1251-抗Tg－McAb与高分化型甲状腺癌细胞结合能力的影响作用．结果显示，Tg的影响与其浓度密切相关，Tg浓度＜200ng／ml时，1251-抗Tg－McAb与高分化状腺甲癌细胞的结合率没有明显影响作用（P＞0．05）；当Tg浓度＞500ng／ml时，影响作用显著（P＜0．01）．偏酸环境可使抗Tg－McAb与高分化型甲状腺癌细胞的结合率稍低，但影响作用不大（P＞0．05），偏碱条件下，标记McAb活性损失增大，结合率显著降低（P＜0．01）．KI和青霉素加地塞米松对结合能力无任何影响．

Effects of various Tg concentrations and pH value on the binding of 125 I-Tg-McAb(18A1 and 18A11 ) todifferentiated thyroid carcinoma cells were investigated in vitro. Similarly,influence of potassium iodine,peni-cillin and dexamethasone were also observed. The results demonstrated that no effect of Tg(＜200 ng/ml)onthe binding of McAb 18A1 and 18A11 to differentiated thyroid carcinoma cells was observed, but significanteffect (P＜0.01 ) was observed when Tg was in a concentration of 500 ng/ml. A low binding percentaage ofanti-Tg-McAb to diffrentiated thyroid carcinorna cells was investigated under pH 6. 8 condition. Ori the con-trary,no significant effects on the binding of McAb 18A1 and 18A11 to diffrentiated thyroid carcinoma wereobserved in the presence of potassium iodide,penicillin or dexamethasone.