体液中毒物与药物快速定性定量分析方法研究Ⅶ.RP—HPLC法测定血清中吗氯贝胺

Studies on Rapid Analysis of Toxicants and Drugs in Body Fluids Ⅶ.RP-HPLC Determination of Meclobemide in Serum

采用反相高效液相色谱法测定人血清中吗氯贝胺（ｍｏｃｌｏｂｅｍｉｄｅ）。血清样品在碱性条件下用无水乙醚提取，有机相在氮气下吹干，残渣用１００μＬ的流动相溶解。ＨＰＬＣ的流动相为：甲醇—水—冰醋酸—四甲基乙二胺（７０３００．３５０．４４），２５４ｎｍ检测。吗氯贝胺的线性范围为１０～２４００μｇ／Ｌ，ｒ＝０．９９９４，平均回收率１０１．３％，ＲＳＤ为２．５５％～６．３１％，最低检测浓度为４μｇ／Ｌ。本法操作简单、快速、准确。

A rapid specific and sensitive HPLC method for the determination of meclobemide in serum was developed.The mobile phase was methanol-water-TMEDA-glacial acetic acid(70∶30∶0 44∶0 35).Alkalinized sample was extracted with anhydrous ether.The organic phase was separated and evaporated to dryness under a stream of nitrogen.The residue was redissolved in the mobile phase and detected at 254nm.The linear range was from 10 to 2400μg/L,r=0 9994.The average recovery was 101 3%,the relative standard deviation was from 2 55% to 6 31%.The useful method for human pharmacokinetic studies was developed.