高效毛细管电泳分离海南新碱衍生物HH07A异构体研究

Separation of Enantiomers of Hainanensine Derivative HH07A by High Performance Capillary Electrophoresis

运用高效毛细管电泳分离了海南新碱衍生物ＨＨ０７Ａ异构体，其分离条件为４×１０－２ｍｏｌ／ＬＴｒｉｓ－Ｈ３ＰＯ４（ｐＨ＝３．６）－６．５×１０－３ｍｏｌ／Ｌβ－环糊精，并用该法初步探讨ＨＨ０７Ａ２个异构体在体外肝微粒体中代谢的立体选择性。

A method for the separation of HH07A enantiomers by high performance capillary electrophoresis was developed.The working solution is composed of 0 04 mol/L tris-H 3PO 4(pH3 6)-0 0065mol/L β cyclodextrin.The method has been used to examine the HH07A metabolism in rat liver microsome system.