摘要
自体外周血造血干细胞（ＰＢＳＣ）移植后血液系统的重建与采集和保存的ＰＢＳＣ的质量和数量有密切关系。在１５名病人的７６次ＰＢＳＣ的采集和保存中，发现ＰＢＳＣ的质量和数量有明显的个体差异，与疾病的类型和化疗方案有密切关系。ＮＨＬ中ＣＤ＋３４细胞与ＣＦＵ－ＧＭ的比例平均为２５％，ＣＭＬ为６７％，ＭＭ最低为１３９％，这可能是因为ＭＭ病人的血清中存在白细胞分化的抑制因子。ＣＭＬ病人中ＣＤ＋３４／ＣＤ－３３细胞在总的ＣＤ＋３４细胞中的比例很低，提示此类病人的早期造血干细胞很少。ＮＩＣＯＯＬ程控降温仪与－８０℃冰箱在细胞的冷冻保存中没有明显的差异。认为单一项目的检测不能全面评价ＢＰＳＣ的质量和数量，应该同时进行单个核细胞计数、ＣＦＵ－ＧＭ数和ＣＤ＋３４细胞数的评估。
The quality and quantity of peripheral blood stem cells (PBSC) are considered as an essential factor for short term and long term hematopoietic reconstruction after PBSC autograft. In 15 patients, 76 apheresis were taken. We found that the quality and quantity of PBSC were different and depend on the type of diseases and the intensity of chemotherapy. The ratio between CD + 34 cells and CFU GM was 25% in Non Hodgkin`s Lymphoma, 67% in Chronic Myelogenous Leukemia and 139% in Multiple Myloma. The lowest ratio was in Multiple Myeloma, it was probably because there was inhibitor in serum of Multiple Myeloma. The CD + 34 /CD - 33 cells in total CD + 34 cells were low for Chronic Myelogenous Leukemia and suggested that few immature stem cell existed in PBSC of CML. The PBSC freezing with the method between Nicool freezer and -80℃ refrigerator was not different. The result demonstrated that only one test was not enough for measure of quality and quantity of BPSC. It is necessary that CFU GM, rate of CD + 34 cells and numbers of MNC be evaluated together.
出处
《白血病》
1997年第3期136-139,共4页
