92例大学生耐卡那霉素肠球菌的分离鉴定及其相关因素

Identification of Kanamycin-Resistant Enterococci in 92 Undergraduates

目的分离鉴定健康大学生粪便中的耐卡那霉素肠球菌,并调查相关因素。方法用卡那霉素ELB选择培养基分离培养92份健康大学生粪便中的肠球菌,调查他们的健康状况及生活习惯等。结果共鉴定出21例携带卡那霉素抗性肠球菌,71例阴性。耐卡那霉素肠球菌的分离率与饮食辛辣的相关性具有统计学意义("2=5.75,P<0.05);而与消化不良、胃肠不适和便秘的相关性无统计学意义("2<3.84,P>0.05)。结论耐卡那霉素肠球菌的分离率与饮食辛辣相关,与消化不良、胃肠不适和便秘等无相关性,其机制及其临床意义有待进一步的研究和随访。

Objective To examine the risk factors associated with the isolation of kanamycin-resistant enterococci from fecal specimens. Methods 92 undergraduates were included in this study. Kanamycin ELB selective medium was used for the cultivation and isolation of enterococci from the fecal specimens. Their health status and living habits were also recorded. Results Among the 92 samples, 21 cases of kanamycin-resistant enterococci were identified. The presence of kanamycin-resistant enterococci was associated with the consumption of spicy foods, and the result was significant （X2=5.75, P〈0.05 ）. Significant association between the presence of kanamycin-resistant enterococci and gastrointestinal discomfort,dyspepsia and constipation was not observed （x^2 〈3.84,P 〉0.05）. Conclusion Consumption of spicy foods may be a risk factor of acquiring kanamycin-resistant enterococci. The mechanisms of acquisition of kanamycin-resistant enterococci are unknown.